Lactose-Inducible System for Metabolic Engineering of Clostridium ljungdahlii
نویسندگان
چکیده
منابع مشابه
Lactose-inducible system for metabolic engineering of Clostridium ljungdahlii.
The development of tools for genetic manipulation of Clostridium ljungdahlii has increased its attractiveness as a chassis for autotrophic production of organic commodities and biofuels from syngas and microbial electrosynthesis and established it as a model organism for the study of the basic physiology of acetogenesis. In an attempt to expand the genetic toolbox for C. ljungdahlii, the possib...
متن کاملConstruction and characterization of a lactose-inducible promoter system for controlled gene expression in Clostridium perfringens.
Clostridium perfringens is a Gram-positive anaerobic pathogen which causes many diseases in humans and animals. While some genetic tools exist for working with C. perfringens, a tightly regulated, inducible promoter system is currently lacking. Therefore, we constructed a plasmid-based promoter system that provided regulated expression when lactose was added. This plasmid (pKRAH1) is an Escheri...
متن کاملCharacterizing acetogenic metabolism using a genome-scale metabolic reconstruction of Clostridium ljungdahlii
BACKGROUND The metabolic capabilities of acetogens to ferment a wide range of sugars, to grow autotrophically on H2/CO2, and more importantly on synthesis gas (H2/CO/CO2) make them very attractive candidates as production hosts for biofuels and biocommodities. Acetogenic metabolism is considered one of the earliest modes of bacterial metabolism. A thorough understanding of various factors gover...
متن کاملMetabolic engineering of Saccharomyces cerevisiae for lactose/whey fermentation.
Lactose is an interesting carbon source for the production of several bio-products by fermentation, primarily because it is the major component of cheese whey, the main by-product of dairy activities. However, the microorganism more widely used in industrial fermentation processes, the yeast Saccharomyces cerevisiae, does not have a lactose metabolization system. Therefore, several metabolic en...
متن کاملExpanding the molecular toolkit for the homoacetogen Clostridium ljungdahlii
Increasing interest in homoacetogenic bacteria for the production of biochemicals and biofuels requisites the development of new genetic tools for these atypical production organisms. An attractive host for the conversion of synthesis gas or electricity into multi-carbon compounds is Clostridium ljungdahlii. So far only limited achievements in modifying this organism towards the production of i...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2014
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.03666-13